Birthdating

Birth dating of cells is important to fully elucidate when a cell has been created, to determine what factors or conditions may have led to its conception.

Birth dating has been applied within many cell constructs, though recent attention has focused on cells within the central nervous system.

H.); Institute for Physiological Chemistry and Pathobiochemistry, Westfalian Wilhelms University, Münster, Germany (R. B.); Center for Accelerator Mass Spectrometry, Livermore, CA (B. Michael’s Hospital, Labatt Family Centre of Excellence in Brain Injury and Trauma Research, Keenan Research Centre of the Li Ka Shing Knowledge Institute of St. Collagen quantity and purity were assessed by SDS-PAGE.

Within the paradigm of neurogenesis, it is extremely important to understand when a functional neuron has been created.

We use this strategy to determine the age of cells in the cortex of the adult human brain and show that whereas nonneuronal cells are exchanged, occipital neurons are as old as the individual, supporting the view that postnatal neurogenesis does not take place in this region.

Retrospective birth dating is a generally applicable strategy that can be used to measure cell turnover in man under physiological and pathological conditions.

However, all 4 markers overlap in their expression pattern to varying degrees.

Here we demonstrate with bromodeoxyuridine birthdating that cells labeled with any 1 of these molecular subplate markers are indeed generated at E11.5 or E12.5 in the mouse.

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